TESTING

Nucleic acid extractionsNucleic Acid Extraction: Nucleic acid extractions from FFPE tissues, Fresh Frozen tissues, and assorted archival materials are performed using several different methodologies including silica based spin columns, magnetic based beads, and organic solvents.

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4 color negativeFour Color Lung Cancer Panel By FISH:  A novel four color PDGFRα/KIT/CC4/RET FISH probe set providing coverage of the PDGFRα, KIT and RET genes.

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FISH SquareMET Amplification by FISH: c-Met amplification activates downstream pathways of the mitogen-activated protein kinase (MAPK) pathway and is a therapeutic target in NSCLC.

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PCR SquareMET Exon 14 Deletion by PCR: c-Met encodes a receptor tyrosine kinase that is activated by its cognate ligand hepatocyte growth factor (HGF). Phosphorylation of c-Met activates downstream pathways of mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K) and phospholipase C (PLC).  Exon 14 is located in the juxtamembrane domain of c-Met and somatic deletion of exon 14 leads to constitutive kinase activation and oncogenesis (Kong-Beltran, et al., 2006). Alternatively spliced variants of Met are found in some gastric and lung carcinomas and may be of prognostic value. In patients with non-small cell lung cancer, MET mutations and amplifications occur in roughly 4-8% and 2-4% respectively.    Read More

 

 

NGS squareNSCLC Targeted Sequencing Panel by NGS: Sequencing of uniquely indexed target enriched libraries is performed using an Illumina MiSeq instrument with bioinformatics analysis through an assay specific pipeline.

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