Nucleic Acid Extraction: Nucleic acid extractions from FFPE tissues, Fresh Frozen tissues, and assorted archival materials are performed using several different methodologies including silica based spin columns, magnetic based beads, and organic solvents.
Four Color Lung Cancer Panel By FISH: A novel four color PDGFRα/KIT/CC4/RET FISH probe set providing coverage of the PDGFRα, KIT and RET genes.
MET Amplification by FISH: c-Met amplification activates downstream pathways of the mitogen-activated protein kinase (MAPK) pathway and is a therapeutic target in NSCLC.
MET Exon 14 Deletion by PCR: c-Met encodes a receptor tyrosine kinase that is activated by its cognate ligand hepatocyte growth factor (HGF). Phosphorylation of c-Met activates downstream pathways of mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K) and phospholipase C (PLC). Exon 14 is located in the juxtamembrane domain of c-Met and somatic deletion of exon 14 leads to constitutive kinase activation and oncogenesis (Kong-Beltran, et al., 2006). Alternatively spliced variants of Met are found in some gastric and lung carcinomas and may be of prognostic value. In patients with non-small cell lung cancer, MET mutations and amplifications occur in roughly 4-8% and 2-4% respectively. Read More
NSCLC Targeted Sequencing Panel by NGS: Sequencing of uniquely indexed target enriched libraries is performed using an Illumina MiSeq instrument with bioinformatics analysis through an assay specific pipeline.